Education
Biotechnology - Micro Biology, Molecular Biology, Technology
Biotechnology is a young science, We used Biology for long long time and when we start using biology as technology rapidly and disruptively in the 1970`s and 1980`s a modern branch of science Biotechnology Born.
Precisely In 1976, a scientist called Robert A Swanson
In 1976, Robert A. Swanson helped launch the birth of biotech when he co-founded Genentech, which became a pioneer in the field of recombinant DNA technology. By creating novel DNA sequences in the lab, Genentech was able to synthesize human insulin for diabetics (1982), and create growth hormones for kids who suffered from a hormone deficiency (1985).
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Accordion Title
Accordion Biochemistry :
Ø Concepts and Scope of Biochemistry
Ø Basics of Biomolecules – Carbohydrates, Proteins, Lipids and Nucleic acids.
Ø General Laboratory Operations, Basic Discipline & Instrument Handling.
Ø Preparations of different Solutions & Buffers. Determination of Molarity and Normality.
Ø Determination of pH and different operations of pH meters.
Ø Estimation of urea by diacetylmonoxime method
Ø Estimation of creatinine by jaffe s method.
Ø preparation of different assays
Ø Centrifugation – Basic Principles sedimentation of Macromolecules sedimentation of velocity sedimentation of Equilibrium.
Ø Types of Centrifuge – Micro centrifuge, High speed centrifuge, Ultra centrifuge, Preparation Centrifuge, Differentiate centrifuge and Density Centrifuge.
Ø Protein Purification – Application and Working Principle of TLC & Paper chromatography.
Ø Phytochemical Analysis by color test using plant extract.
Microbiology:
Basic Pattern:
Ø Over view of Microbiology – Concept & Scope.
Ø General Laboratory Operations
Ø Microscopy – Description and Operation of Compound Microscope use oil immersion objective.
Ø Sterilization Methods – Operation of Autoclave, hot air oven, Culture fumigation & surface sterilization.
Ø Culture media preparation – Nutrient Broth, Nutrient Agar, Potato Dextrose agar and slant preparation for culture.
Ø Isolation techniques – pure culture by streak plate method.
Ø Stain & Staining – Principles of staining, simple staining and differentiation staining.
Ø Aseptic techniques – Culture transfer from solid to solid, solid to liquid and liquid to liquid.
Ø Different culture techniques – Streak Plate, Pour Plate and Spread Plate.
Ø Antibiotic Sensitivity Test – by paper disc method.
Ø Antimicrobial Activity – Antibacterial activity from plant extract by using non pathogen strains. Antifungal activity from plant extract by using non pathogen strains.
Ø Study of Endophytic fungi and Isolation method by using PDA medium.
Cell & Molecular Biology:
Advanced pattern:
Ø Concepts of cell biology – basic functional unit of living cells.
Ø Cellular classification – different domains, Cell organization – Prokaryotic & Eukaryotic cells.
Ø Determination of E.coli bacteria from Buccal smear method.
Ø Composition of different buffer preparation and micro pipette handling
Ø Application and working methodology of Agarose gel electrophoresis.
Ø Isolation of genomic DNA from plant samples through Agarose gel electrophoresis.
Ø Isolation of genomic DNA from Animal sample through Agarose gel electrophoresis.
Ø Isolation of genomic DNA from bacterial samples through Agarose gel electrophoresis.
Ø Isolation of genomic DNA from human blood sample through Agarose gel electrophoresis.
Ø PCR – working methodology & Concepts.
NanoBiotechnology:
General Pattern:
Ø Development of Nanobiotechnolgy – Progress and overview
Ø Application in Nanobiology by different fields.
Ø General Laboratory Operations (GLO)
Ø Nanoparticles synthesis from different metals (Ag and Cu) by chemical approaches.
Ø Biosynthesis – Biological Nanoparticles production – plants & Microbes.
Ø Green synthesis of nanopartticles from plant extract by using eco friendly metals (Ag and Cu).
Ø Biological synthesis of nanoparticles from microbial extract by using (Ag and Cu).
Ø CharacterizationContent